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Accell siRNA achieves what no other product can claim: delivery into difficult-to-transfect cells without additional reagents, virus, or instruments. Achieve gene silencing in cells that had been beyond the reach of conventional RNAi methods due to toxicity from transfection reagents or undesirable viral responses.
This breakthrough in siRNA delivery requires no transfection reagent, but has some unique application requirements.
Due to the unique nature of Accell siRNA delivery, it requires a higher working concentration than conventional siRNAs. This table provides the approximate number of reactions (wells) at recommended 1μM Accell siRNA working concentration in different plate formats (assuming no loss from pipetting).
Thermo Scientific offers three complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
Click the data figures below to learn more about Accell siRNA reagents.
Cell types demonstrating effective silencing with Accell siRNA
The Accell siRNA application protocol simplifies targeted gene knockdown
Accell siRNA delivery and gene silencing in cardiomyocytes
Internal validation and peer-reviewed publications report numerous successes with difficult-to-transfect cell types.
(A) Combine Accell siRNA with Accell delivery media (or other low- or no-serum media) (B) Add Accell delivery mix directly to cells and incubate for 72 hours.
Neonatal rat ventricular myocytes were incubated with 1 μM Accell Green (A; Cat# D-001950-01) or Red (B; Cat# D-001960-01) Non-targeting siRNA for 72 hours in Accell delivery media (Cat# B-005000). Nuclei were stained with DAPI (blue). Labeled control uptake showed diffuse cytoplasmic localization in nearly all cells. The bar graph indicates the level of gene silencing achieved with Accell GAPD Control siRNA (Cat# D-001930-03) and Pool (Cat# D-001930-30) control reagents when used with neonatal rat ventricular myocyte (NRVM) media or Accell delivery media. Myocytes were prepared as described in Maass AH & Buvoli M. Cardiomyocyte preparation, culture, and gene transfer. Methods in Molecular Biology 2007;366: 321-30. mRNA expression was determined by QuantiGene branched DNA assay (Panomics).