Accell siRNA

Accell siRNA achieves what no other product can claim: delivery into difficult-to-transfect cells without additional reagents, virus, or instruments. Achieve gene silencing in cells that had been beyond the reach of conventional RNAi methods due to toxicity from transfection reagents or undesirable viral responses.

Highlights

• Accell siRNA enters cells without the need for transfection reagents, virus (or viral vectors), or instruments.
• Novel siRNA modifications facilitate uptake, stability, specificity and knockdown efficiency.
• Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types.

Experimental considerations

This breakthrough in siRNA delivery requires no transfection reagent, but has some unique application requirements.

• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1µM working concentration.
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended.
• Full-serum media can be added back after 48 hours of incubation, optimal mRNA silencing is typically achieved by 72 hours, or up to 96 hours for protein knockdown.

SMARTpool:

• A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity.

Set of 4:

• A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene.

Set of 4 Upgrade:

• Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.

Individual siRNAs:

• Select 1, 2, 3 or 4 individual siRNAs per gene.

Due to the unique nature of Accell siRNA delivery, it requires a higher working concentration than conventional siRNAs. This table provides the approximate number of reactions (wells) at recommended 1μM Accell siRNA working concentration in different plate formats (assuming no loss from pipetting).

Which siRNA is right for you?

Thermo Scientific offers three complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.

	Cost-effective, efficient silencing	High specificity for reduced off-targets + efficient silencing	Target silencing in difficult-to-transfect cells
	siGENOME siRNA	ON-TARGETplus siRNA	Accell siRNA
Pre-designed for Human, Mouse and Rat protein-coding genes	+	+	+
Recommended for transfectable mammalian cells in culture	+	+
Recommended for neuronal, suspension, primary and other difficult-to-transfect cells			+
Recommended transfection reagent	DharmaFECT transfection reagent	DharmaFECT transfection reagent	None required
Available as SMARTpool reagent	+	+	+
Available as four individual siRNAs	+	+	+
New 2 nmol size available for individual siRNA	+	+
Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs	+	+
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake	Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading	+	+
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity		+	+
Modifications to facilitate cellular uptake without separate transfection reagents			+
Stabilizing modifications to prevent nuclease-mediated degradation			+
Sequence information provided with purchase	+	+	+

Click the data figures below to learn more about Accell siRNA reagents.