Custom Synthesis Overview

Catalog	Item	Price	Quantity	Subtotal
Cart is Empty

Overview
2'-ACE Chemistry
Scale and Yield
Quality Control

Custom Single-stranded RNA
Single-stranded RNA Synthesis	Order
Multiple Single-stranded RNA Syntheses	Order
Custom siRNA
siRNA Synthesis	Order
Modified siRNA Synthesis	Order
Multiple siRNA Syntheses	Order
Custom SMARTpool Design & Synthesis	Order
Custom microRNA
Custom microRNA	Request Quote
siDESIGN Center
siRNA Design Tool	Design Now

Single-stranded RNA

Design a single-stranded RNA molecule with a wide variety of chemical modifications, learn about our capabilities for long RNA oligos, dye labeling, custom amidites and more.

Learn about customizing your siRNA with proprietary modification patterns or other chemical modifications, investigate your options for in vivo RNAi, design your own siRNA using the siDESIGN Center, learn about custom SMARTpool options and more.

microRNA

Customize a Thermo Scientific miRIDIAN Mimic or Hairpin Inhibitor, or design your own microRNA reagent with specialized chemical modifications, in vivo processing, gram quantities and more.

2'-ACE Chemistry

RNA applications can be executed with greater ease and confidence by utilizing the powerful advantages of the Thermo Scientific 2'-ACE technology.

Greater reproducibility than any other chemistry
Crude yields of unmatched purity without additional purification time and expense
Maximum sequence fidelity and integrity due to enhanced coupling efficiency

Download the Tech Note 2'-ACE RNA Synthesis Chemistry for more information on the details of the Thermo Scientific RNA synthesis platform.

All synthetic RNA oligos must be chemically protected at multiple sites during synthesis, regardless of the chemistry platform used. However, Thermo Scientific patented 2'-ACE synthesis chemistry allows for the quickest and mildest deprotection conditions compared to other chemistries like 2'-tBDMS and "2'-TOM. Short deprotection times and a mild chemical environment promote the highest level of purity for synthetic RNA in the marketplace today.

For unmodified, unpurified RNA, the following approximate percentages of full-length material can be expected:

	2'-ACE	2'-tBDMS	2'-TOM
ASCE*	>99%	>98%	95%
21-mer **	81%	65%	34%
50-mer **	61%	36%	8%
Coupling reaction	Fast	Slow	Slow
Deprotection conditions	Aqueous	Organic	Organic
Product can be delivered in 2'-protected or deprotected state	Yes	No	No

click to enlarge

Figure 1. Thermo Scientific 2'-ACE chemistry features superior RNA synthesis quality.

click to enlarge

Figure 2. Sequence fidelity and high yields are the hallmarks of 2'-ACE chemical synthesis

*The average stepwise coupling efficiencies (ASCE) for each method.
**Percent full-length material listed above are approximate; exact yields will depend on sequence composition.

Scale and Yield

Thermo Fisher Scientific offers a wide variety of synthesis scales to match your quantity preferences.

What is the difference between scale and yield?

SCALE

Relative amount of starting material packed into an RNA synthesis column
Typically expressed as micromole (µmol) increments

YIELD

Final amount of material delivered
Typically expressed as nanomole (nmol), micromole (µmol), milligram (mg), or gram (g) amounts

SCALE ≠ YIELD

Actual yields obtained for the final product may range from 10%-90% of the scale, depending on the oligo specifications (i.e. modification type, length, purification process, etc.).

If you have a minimum yield requirement for your experiments, please contact a Technical Support Scientist for recommendations on which scale to select for your online order.

Available synthesis scales can accommodate microgram to multi-gram quantities:

	Synthesis scales available online	Synthesis scales available by request
siRNA	0.025 µmol - 10 µmol	20 µmol - 2 mmol
Single-stranded RNA	0.05 µmol - 1 µmol	2 µmol - 2 mmol

For unmodified siRNA, the following approximate yields can be expected:

	Standard (A4)**		Purified		in vivo		in vivo HPLC
	nmol	mg	nmol	mg	nmol	mg	nmol	mg
0.025 µmol scale	20	0.25	-	-	-	-	-	-
0.05 µmol scale	40	0.5	20	0.25	-	-	-	-
0.2 µmol scale	150	2	80	1	100	1.3	50	0.65
0.4 µmol scale	300	4	160	2	200	2.6	100	1.3
1.0 µmol scale	750	10	320	4	500	6.6	200	2.6
2.0 µmol scale	1500	20	640	8.5	1000	13	500	6.6
5.0 µmol scale	3750	50	1600	21	2505	33	1000	13
10.0 µmol scale	7500	100	3200	42	5000	66	2500	33

For single-stranded RNA, yields may vary greatly depending on the specifications of your request (oligo length, sequence, modifications, purification, ect.). Please contact Technical Support for RNA yield estimates at ts.molbio@thermofisher.com or +1 800 235 9880.

*Yields reported above should be considered estimates, and are not guaranteed amounts. Please inquire within if a minimum guaranteed amount is required.
"**Standard processing includes 2'-ACE deprotection, desalting, and annealing.

Quality Control

At Thermo Fisher Scientific, we pride ourselves on our reputation for high quality RNA synthesis.

As part of our long-standing commitment to quality, we employ the following standards for quality assurance:

All RNA synthesis products (including siRNAs) are analyzed by mass spectrometry and quantified by UV spectroscopy
MALDI TOF MS for all standard siRNAs and single-stranded RNAs
ESI MS for all modified siRNAs and all longer or highly modified RNAs
HPLC or PAGE for purification of RNA products
UPLC or CGE for quality analysis when appropriate
Custom QC methods and documentation are available upon request

See below for definitions

Quality control documentation available upon request includes:

Mass spectrometry traces
UPLC or CGE analysis
Duplex or individual strand purity analysis
Certificate of Analysis
Endotoxin certification
Custom QC documentation

For all mid-scale synthesis projects (200 mg - 10 g), a stringent manufacturing process is adhered to.

HPLC purification highly recommended to achieve optimal purity
Batch records maintained on all synthesis projects (we follow GMP guidelines, but are not GMP certified)
Endotoxin testing included on all mid-scale orders
Data deliverables include MS, UPLC, endotoxin results, and a Certificate of Analysis

Definitions:

MALDI TOF MS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
ESI MS: Electrospray ionization mass spectrometry
HPLC: High performance liquid chrmatography
PAGE: Polyacrylamide gel electrophoresis
UPLC: Ultra performance liquid chromatography
CGE: Capillary gel electrophoresis

Thermo Scientific 2'-ACE chemistry features superior RNA synthesis quality.

Sequence fidelity and high yields are the hallmarks of 2'-ACE chemical synthesis

Unlike other synthesis chemistries, unmodified siRNA or RNA oligos >35 nt synthesized using 2'-ACE chemistry do not require purification prior to in vitro applications.

Comparison of an unmodified, unpurified 36 nt RNA oligo synthesized using 2'-ACE, 2'-tBDMS, and 2'-TOM chemistry platforms. Anion exchange-HPLC traces for (A) 2'-ACE, (B) 2'-tBDMS, and (C) 2'-TOM illustrate a superior level of purity achieved with Thermo Scientific 2'-ACE chemistry. Smaller peaks eluting earlier than the full-length oligo (shaded area) are indicative of incomplete, non full-length, n-x products. Results are typical, but may vary slightly depending on oligo sequence.

Custom Synthesis Overview