Discontinuation of selected Thermo Scientific molecular biology products

In order to better serve our customers and offer best-in-class technologies for their research, we are in the process of refining our molecular biology product offering. As a result, in addition to offering several new products in 2012, a small number of catalog products will be discontinued as of March 31st, 2012.

The majority of the discontinued products have been replaced by an equivalent or superior product, and for many of these replacement products, samples are available. Also note that even though the manufacture of the discontinued products will cease on March 31, 2012, there may be remaining inventory available for sale beyond this date.

Please do not hesitate to contact your local Thermo Scientific representative if you would like to try a sample of a replacement product or if you have any questions. We look forward to the continuing opportunity to serve our customers, and toward assisting you through this product transition.


DNA Polymerases, PCR kits and polymerase buffers
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
F-500S
F-500L		 No DyNAzyme I DNA Polymerase (native) EP0071/EP0072
EP0281/EP0282
EP0401/EP0402/ EP0405/EP0406		 Taq DNA Polymerase (native, with BSA) OR
Taq DNA Polymerase (native, without BSA) OR
Taq DNA Polymerase (recombinant)
F-502S
F-502L		 No DyNAzyme I DNA Polymerase with Mg-free buffer EP0071/EP0072
EP0281/EP0282
EP0401/EP0402/ EP0405/EP0406		 Taq DNA Polymerase (native, with BSA) OR
Taq DNA Polymerase (native, without BSA) OR
Taq DNA Polymerase (recombinant)
F-550S Yes DyNAzyme I DNA Polymerase Kit EP0071/EP0072
EP0281/EP0282
EP0401/EP0402/ EP0405/EP0406		 Taq DNA Polymerase (native, with BSA) OR
Taq DNA Polymerase (native, without BSA) OR
Taq DNA Polymerase (recombinant)
F-503S Yes DyNAzyme II DNA Polymerase with Mg-free buffer EP0401/EP0402/EP0405/EP0406 Taq DNA Polymerase (recombinant)
F-522S
F-522L		 No DyNAzyme II Hot Start Reaction Buffer None
F-508S Yes DyNAzyme II PCR Master Mix K0171/K0172
K1071/K1072		 PCR Master Mix (2X)
DreamTaq PCR Master Mix
F-551S
F-551L		 No DyNAzyme II DNA Polymerase PCR Kit EP0701/EP0702/EP0703
EP0704/EP0705		 DreamTaq DNA Polymerase
F-504S
F-504L		 No DyNAzyme II Hot Start DNA Polymerase EP0601/EP0602/ EP0603
F-122S/F-122L		 Maxima Hot Start Taq DNA Polymerase or Phire Hot Start II DNA Polymerase
F-512S
F-512L		 No DyNAzyme EXT Buffer Pack for Mg Optimization None
F-552S
F-552L		 No DyNAzyme EXT PCR Kit K1081/K1082
F-505S/F-505L		 Long PCR Enzyme Mix (not available in US)
DyNAzyme EXT DNA Polymerase
F-514S
F-514L		 No Optimized DyNAzyme EXT Buffer None
K1091 No Fast Tissue-to-PCR Kit F-140
F-150		 Phire Animal Tissue Direct PCR Kit OR or Phusion Human Specimen Direct PCR Kit
K0211 No PyroStart Fast PCR Master Mix F-548S/F-548L Phusion Flash High-Fidelity PCR Master Mix
AB-0908 Yes Thermo-Start Taq DNA Polymerase EP0601/EP0602/EP0603
F-122S/F-122L		 Maxima Hot Start Taq DNA Polymerase or Phire Hot Start II DNA Polymerase
AB-0938/DC/15A Yes Thermo-Start Taq DNA Polymerase Master Mix K1051/K1052 Maxima Hot Start PCR Master Mix
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qPCR kits and master mixes
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
AB-1318 Yes ABsolute qPCR Low ROX Mix K0261/K0262/K0263 Maxima Probe qPCR Master Mix, ROX solution provided
AB-1132/A Yes ABsolute QPCR Mix K0261/K0262/K0263 Maxima Probe qPCR Master Mix, ROX solution provided
AB-4138/A Yes ABsolute Blue QPCR ROX Mix AB-4139/A Larger pack size of the same ABsolute Blue QPCR ROX Mix
AB-1285/A Yes ABsolute QPCR SYBR Green Capillary Mix K0251/K0252/ K0253 Maxima SYBR Green qPCR Master Mix, ROX solution provided
AB-1322/A Yes ABsolute QPCR SYBR Green Low ROX Mix K0251/K0252/ K0253 Maxima SYBR Green qPCR Master Mix, ROX solution provided
AB-4322/A Yes ABsolute Blue QPCR SYBR Green Low Rox Mix AB-4323/A Larger pack size of the same ABsolute Blue QPCR SYBR Green Low Rox Mix
AB-1162/A Yes ABsolute QPCR SYBR Green ROX (500nM) Mix K0221/K0222/ K0223 Maxima SYBR Green qPCR Master Mix/ROX
F-440S
F-440L		 No DyNamo Capillary SYBR Green 2-step qRT-PCR Kit K1641/K1642
K0251/K0252/K0253		 Maxima First Strand cDNA Synthesis Kit or Maxima™ SYBR Green qPCR Master Mix (2X), ROX solution provided
F-420S Yes DyNAmo Capillary SYBR Green qPCR Kit K0251/K0252/ K0253 Maxima SYBR Green qPCR Master Mix, ROX solution provided
F-410S Yes DyNAmo HS SYBR Green qPCR Kit K0251/K0252/ K0253 Maxima SYBR Green qPCR Master Mix, ROX solution provided
F-460S
F-460L		 No DyNamo Probe 2-step qRT-PCR Kit K1641/K1642
K0261/K0262/K0263		 Maxima First Strand cDNA Synthesis Kit plus
Maxima Probe qPCR Master Mix, ROX in a separate tube
F-450S
F-450XL 		Yes DyNAmo Probe qPCR Kit K0261/K0262/ K0263 Maxima Probe qPCR Master Mix, ROX solution provided
F-430S Yes DyNAmo SYBR Green 2-step qRT-PCR Kit K1641/K1642
K0251/K0252/K0253		 Maxima First Strand cDNA Synthesis Kit plus Maxima SYBR Green qPCR Master Mix (2X), ROX solution provided
F-400S Yes DyNAmo SYBR Green qPCR Kit K0251/K0252/ K0253 Maxima SYBR Green qPCR Master Mix, ROX solution provided
F-400RS Yes DyNAmo SYBR Green qPCR Kit with ROX K0221/K0222/ K0223 Maxima SYBR Green/ROX qPCR Master Mix
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Reverse Transcription
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
EP0641 No AMV Reverse Transcriptase EP0741/EP0742/EP0743 Maxima Reverse Transcriptase
F-570S
F-570L		 No AMV Reverse Transcriptase EP0741/EP0742/ EP0743 Maxima Reverse Transcriptase
K1611 Yes First strand synthesis kit K1621/K1622 RevertAid First Strand cDNA Synthesis Kit
EP0351 Yes M-MuLV Reverse Transcriptase EP0441/EP0442 RevertAid Reverse Transcriptase (not available in Canada)
F-572S
F-572L 		No M-MuLV Reverse Transcriptase RNAse H- (not available in US) EP0451/EP0452 RevertAid H Minus Reverse Transcriptase (not available in US)
F-580S
F-580L 		No Robust I RT-PCR Kit AB-1454/A AB-1454/B
AB-1454/LD/A AB-1454/LD/B
AB-1455/A AB-1455/B 		Verso 1-Step Kit OR
Verso 1-Step Kit Reddymix OR
Verso 1-Step Kit with Thermo-Start
F-590S
F-590L 		No Robust II RT-PCR Kit AB-1454/A AB-1454/B
AB-1454/LD/A AB-1454/LD/B
AB-1455/A AB-1455/B		 Verso 1-Step Kit OR
Verso 1-Step Kit Reddymix OR
Verso 1-Step Kit with Thermo-Start
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Nucleotides
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
F-560S
F-560L
F-560XL		 No dNTP Mix R0191
R0192
R1121		 dNTP Mix
F-561A No dATP solution R0141 dATP
F-561C No dCTP solution R0151 dCTP
F-561G No dGTP solution R0161 dGTP
F-561T No dTTP solution R0171 dTTP
F-561U No dUTP solution R0133 dUTP
F-622 No Ribonucleotide Set R0481 NTP Set
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Restriction Enzymes
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
ER1721 No Aasl (Drdl) FD1724 FastDigest (Aasl)
ER0161 No Cfrl (Eael) None
ER1541 No Ppil None
ER1591 No Bfil (Bmrl) None
ER1601 No Hin4l None
ER1671 No Eco57Ml None
ER1691 No Smul (Faul) None
ER1911 No Tstl None
ER1961 No Bdal None
ER1991 No Tsol None
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Other enzymes
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
F-201S
F-201L 		No CIP, Calf Intestinal Alkaline Phosphatase EF0651
EF0652 		FastAP Thermosensitive Alkaline Phosphatase
F-202S
F-202L 		No Proteinase K EO0491
EO0492 		Proteinase K (recombinant), PCR grade
F-617S
F-617L		 No T7 RNA Polymerase EP0111
EP0112
EP0113 		T7 RNA Polymerase
AB-0325
AB-0325/B		 No Tth DNA Ligase None
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Molecular weight markers
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
SM0111 No Lambda DNA/Eco91I (BstEII) Marker, 15 SM0311 GeneRuler 1 kb DNA Ladder
SM0161 No Lambda DNA/Eco130I (StyI) Marker, 16 SM1331 GeneRuler 1 kb Plus DNA Ladder
SM1051 No Lambda DNA/Eco47I (AvaII) Marker, 13 SM1331 GeneRuler 1 kb Plus DNA Ladder
SM0361 No Lambda DNA/PstI Marker, 24 SM1331 GeneRuler 1 kb Plus DNA Ladder
SM0291 No Lambda-pUC Mix Marker, 4 SM1331 GeneRuler 1 kb Plus DNA Ladder
SM0121 No pBR322 DNA/AluI Marker, 20 SM0371/SM0372
SM0373
SM1133		 GeneRuler 50 bp DNA Ladder
GeneRuler 50 bp DNA Ladder, ready-to-use, 50-1000 bp
O'GeneRuler 50 bp DNA Ladder, ready-to-use, 50-1000 bp
SM0123 No pBR322 DNA/AluI Marker, 20, ready-to-use
SM0301
SM0302 		No pUC Mix Marker, 8
SM0303 No pUC Mix Marker, 8, ready-to-use
SM0261 No ΦX174 DNA/HinfI Marker, 10
SM0231 No Lambda Mix Marker, 19 SM1353 GeneRuler High Range DNA Ladder, ready-to-use, 10, 171-48, 502 bp
SD0401 No pUC19 DNA/SmaI, Digested & Dephosphorylated None
SD0411 No pUC19 DNA/BamHI, Digested & Dephosphorylated None
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Transfection
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
R0511 No ExGen 500 in vitro Transfection Reagent R0531 TurboFect Transfection Reagent
R0521 No ExGen in vivo Transfection Reagent R0541 TurboFect in vivo Transfection Reagent
R1401 No TurboFect siRNA Transfection Reagent T-2001-XX
T-2010-XX 		DharmaFECT 1 Transfection Reagent
DharmaFECT Duo Transfection Reagent
ATR1740
ATR1741
ATR1742
ATR1743 		No Arrest-In Transfection Reagent R0531 TurboFect Transfection Reagent
ETR4620
ETR4621
ETR4622
ETR4623		 No Express-In Transfection Reagent R0531 TurboFect Transfection Reagent
ATR4164
ATR4165
ATR4166
ATR4167		 No Fluorescent Arrest-In Transfection Reagent R0531 TurboFect Transfection Reagent
TLP4614
TLP4691
TLP4692
TLP4615		 No Trans-Lentiviral shRNA Packaging Kits with Arrest-In Transfection Reagent TLP5912,TLP5913
TLP5914, TLP5917		 Trans-Lentiviral shRNA Packaging Kits with Calcium Phosphate Transfection Reagent
TLP4616
TLP4693
TLP4694
TLP4617		 No Trans-Lentiviral ORF Packaging Kits with Express-In Transfection Reagent TLP5916,TLP5919
TLP5920, TLP5915		 Trans-Lentiviral ORF Packaging Kits with Calcium Phosphate Transfection Reagent
RHS5086
RHS5087		 No RNAintro shRNA Transduction Kits containing Arrest-In TBD RNAintro shRNA Transduction Kits containing Calcium Phosphate Transfection Reagent
RHS4287
RHS4741		 No RNAintro shRNA Transfection Kits containing Arrest-In TBD RNAintro shRNA Transfection Kits containing TurboFect Transfection Reagent
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Membranes
Discontinued Cat. No. Any sizes available after March 31? Description Replacement Cat. No. Description
M1001
M1002 		No SensiBlot Plus Nylon Membrane None
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