Previously added items:
Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site ( see Reference1).
Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion ( see Reference8).
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A. P. Feinberg, B. Vogelstein, Addendum to: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem.137 , 266-267 (1984).
H. Yu et al. , Cyanine dye dUTP analogs for enzymatic labeling of DNA probes.Nucleic Acids Res, 22 , 3226-3232 (1994).
G. T. Walker, Empirical aspects of strand displacement amplification.PCR Methods Appl.3 , 1-6 (1993).
F. Sanger et al. , DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. USA.74 , 5463-5467 (1977).
H-M. Eun, Enzymology Primer for Recombinant DNA Technology (Academic Press, Inc., San Diego, California, 1996).
J. M. Clark et al. , Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli . J. Mol. Biol.198 , 123-127 (1987).