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Home | Molecular Biology | Restriction & Modifying Enzymes | DNA and RNA Modifying Enzymes | Nucleases

Micrococcal Nuclease

EDTA inactivation Not inactivated at 80°C in 20 min Recombinant enzyme

Micrococcal Nuclease (S7 Nuclease) is an endo-exonuclease that digests single-stranded and double-stranded DNA and RNA.
  
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Thermo Scientific Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates (see Figure 1 in Supporting Data). The enzyme's activity is strictly dependent on Ca2+.

Applications

  • Hydrolysis of nucleic acids in crude cell-free extracts (see Reference 1)
  • Sequencing of RNA (see​ Reference 2)
  • Studies of chromatin structure (see​ Reference 3)
  • A model for protein folding and for structure-function studies (see​ References 4, 5)
  
Storage Condition-20 C
HazardousNo
Concentration300 U/µL
Definition of Activity Unit
  • One unit of the enzyme releases 1.0 A260 unit of acid-soluble products in 30 min at 37°C.
  • Enzyme activity is assayed in the following mixture: 100 mM sodium-glycine (pH 8.6), 10 mM CaCl2, and 4.5 mM calf thymus DNA.
InactivationInactivated by the addition of EGTA or EDTA.
InhibitionInhibitors: metal chelators, deoxythymidine 3' 5'-diphosphate.
Molecular Weight16.9 kDa monomer
Quality Control95% purity confirmed by SDS-PAGE.
SourceE. coli cells with a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).
Storage BufferThe enzyme is supplied in: 20 mM Tris-HCl (pH 7.6), 50 mM NaCl, and 50% (v/v) glycerol.
Micrococcal nuclease activity

Micrococcal nuclease activity

Micrococcal nuclease activity

Micrococcal nuclease activity.


References

  1. H. R. B. Pelham, R. J. Jackson, An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67, 247-256 (1976).
  2. G. Krupp, J. H. Gross, Rapid RNA sequencing: nucleases from Staphylococcus aureus and Neurospora crassa discriminate between uridine and cytidine. Nucleic Acids Res. 6, 3481-3490 (1979).
  3. D. J. Telford, B. W. Stewart, Micrococcal nuclease: its specificity and use for chromatin analysis. Int. J. Biochem. 21, 127-137 (1989).
  4. P. W. Tucker et al., Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. IV. The nuclease as a model for protein folding. Molec. Cell. Biochem. 23, 131-140 (1979).
  5. D. A. Torchia et al., Staphylococcal nuclease: sequential assignments and solution structure. Biochemistry 27, 5509-5524 (1989).

Protocols and Product Manuals

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