Previously added items:
Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis (see Reference 8).
Use of this enzyme in certain applications may be covered by patents and may require a license.
Unbiased amplification of human genomic DNA with phi29 DNA Polymerase.100 copies (0.33 ng) of human genomic DNA were amplified with phi29 DNA Polymerase according to the procedure of Dean et al., 2002 (see Reference 9). Radioactive [α-33P]-dATP incorporation into the DE-81 adsorbable form was calculated to quantify amplification of total DNA. Amplification efficiency of specific genes located in different chromosomes was determined by real-time PCR using the ABI 7700.