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Thermo Scientific RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues (see Figure 1 in Supporting Data).
It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (see References 1, 2).
Recommended concentration of RNase A is 1 to 100 µg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA (see Reference 9).
RNase A activity.
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R. T. Raines, Ribonuclease A. Chem. Rev. 98, 1045-1065 (1998).
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R. C. Sharma et al., A rapid procedure for isolation of RNA-free genomic DNA from mammalian cells. BioTechniques. 14, 176-178 (1993).
R. M. Myers et al., Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science. 230, 1242-1246 (1985).
E. Winter et al., A method to detect and characterize point mutations in transcribed genes: Amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells. Proc. Natl. Acad. Sci. USA 82, 7575-7579 (1985).
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