Previously added items:
Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides (see Figure 1 in Supporting Data). It is five times more active on DNA than on RNA (see Reference 1). S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.
The enzyme is a glycoprotein with carbohydrate content of 18%.
S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (see Reference 6).
Nuclease S1 activities.