Home | Molecular Biology | Molecular Biology Applications | Molecular Cloning | Molecular Cloning Enzymes

S1 Nuclease

Thermal inactivation at 70°C in 10 min in the presence of EDTA

S1 Nuclease is an endonuclease which degrades single-stranded DNA and RNA.
  
Loading...

Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides (see Figure 1 in Supporting Data). It is five times more active on DNA than on RNA (see Reference 1). S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.

The enzyme is a glycoprotein with carbohydrate content of 18%.

Applications

  • Removal of single-stranded overhangs of DNA fragments (see​ Reference 2)
  • S1 transcript mapping (see​ References 3, 4)
  • Cleavage of hairpin loops
  • Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (see​ Reference 5)

Note

S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (see​ Reference 6).

  
Storage Condition-20 C
HazardousNo
5X Reaction Buffer200 mM sodium acetate (pH 4.5 at 25°C), 1.5 M NaCl, and 10 mM ZnSO4.
Concentration100 U/µL
Definition of Activity UnitOne unit of the enzyme produces 1 µg of acid soluble deoxyribonucleotides in 1 min at 37°C.
InactivationInactivated by heating at 70°C for 10 min in the presence of EDTA
InhibitionInhibitors: metal chelators, PPi, Pi, 5'-ribonucleotides, and deoxyribonucleotides
Molecular Weight29 kDa monomer
Quality Control
  • The absence of contaminating double-stranded DNA specific nuclease activity confirmed by appropriate quality test.
  • Functionally tested for the generation of unidirectional deletions in DNA.
SourceAspergillus oryzae
Storage BufferThe enzyme is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM ZnCl2, and 50% (v/v) glycerol.
Nuclease S1 activities

Nuclease S1 activities

Nuclease S1 activities

Nuclease S1 activities.


References

  1. R. I. Lehman, Endonucleases specific for single-stranded polynucleotides. The Enzymes (Boyer, P.D., ed. 3, 1981). 4, 193-201.
  2. T. M. Roberts et al., A general method for maximizing the expression of a cloned gene. Proc. Natl. Acad. Sci. USA. 76, 760-764 (1979).
  3. A. J. Berk, P. A. Sharp, Spliced early mRNAs of simian virus 40. Proc. Natl. Acad. Sci. USA. 75, 1274-1278 (1978).
  4. U. Weidle, C. Weissmann, The 5’-flanking region of a human IFN-alpha gene mediates viral induction of transcription. Nature. 303, 442-446 (1983).
  5. S. Henikoff, Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 28, 351-359 (1984).
  6. V. M. Vogt, Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur. J. Biochem. 33, 192-200 (1973).

MSDS & Certificates

pdf   S1 Nuclease MSDS
pdf   MSDS (JP) - S1 Nuclease

Product Guides and Selectors

pdf   S1 Nuclease Product Information