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Thermo Scientific CpG Methylated Jurkat Genomic DNA is human male genomic DNA from Jurkat cells, enzymatically methylated with CpG Methyltransferase (M.SssI). Genomic DNA was isolated from Jurkat E6.1 (human acute T-cell leukemia, clone E6.1) cells grown to confluency in RPMI media supplemented with 10% bovine fetal serum. Isolated genomic DNA was treated with CpG Methyltransferase, repurified, and dissolved in TE buffer. ≥ 98% CpG methylation level confirmed by bisulfite sequencing.