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5-Fluoroorotic Acid

Formula: C5H3FN2O4 X H2O, Molecular weight: 192.1

Store at -20°C

5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase.
  
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The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).

Applications

  • Detection of URA3 gene expression in yeast
  • Construction of yeast 2-hybrid libraries (see Reference 1)
  • Other areas of yeast genetics including gene replacement, analysis of transposable genetic elements, and identification of gene mutations (see​ Reference 2)
Convertion of 5-FOA to fluorodeoxyuridine

Convertion of 5-FOA to fluorodeoxyuridine

5-Fluoroorotic Acid Chemical Structure

Note

  • Low solubility in water
  • Solubility in dimethylsulfoxide is greater than 150 mg/mL
  • Preparation of a 100 mg/mL solution in dime-thylsulfoxide is recommended
  
Storage Condition-20 C
HazardousNo
FormulaC5H3FN2O4 x H2O
Molecular weight192.1
Quality ControlPurity of 98%.
StorageStore at -20°C in the dark.

References

  1. P. L. Bartel, S. Fields, Yeast 2-Hybrid System (Oxford University Press, New York, 1997), vol. 7, pp. 109-147.

  2. C. Kaiser et al., Methods in Yeast Genetics (Cold spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994).

MSDS & Certificates

pdf   5-Fluoroorotic Acid - MSDS

Protocols and Product Manuals

pdf   5-Fluoroorotic Acid - Product Information