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The Thermo Scientific 5-Fluoroorotic Acid is a yeast with an active URA3 gene (Ura+) converts 5-FOA to fluorodeoxyuridine, which is toxic to cells (see the scheme below). Yeast strains carrying a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. 5-FOA is frequently used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and E. coli (pyrF).
Convertion of 5-FOA to fluorodeoxyuridine
P. L. Bartel, S. Fields, Yeast 2-Hybrid System (Oxford University Press, New York, 1997), vol. 7, pp. 109-147.
C. Kaiser et al., Methods in Yeast Genetics (Cold spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994).