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Aminoallyl-dUTP

Formula: C12H17N3O14P3Na3, Molecular weight: 589.2 (acid form: 523.2)

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Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is a modified nucleotide used to enzymatic indirect non-radioactive labeling of DNA.
  
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Aminoallyl-dUTP chemical structure

Aminoallyl-dUTP
Formula: C12H17N3O14P3Na3
Molecular weight: 589.2 (acid form: 523.2)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications

  • Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension
  
Storage Condition-20 C
HazardousNo
FormulaC12H17N3O14P3Na3 
General Characteristicslambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0)

lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
Molecular weight589.2 (acid form: 523.2)
Quality Control
  • Functionally tested in cDNA synthesis with RevertAid H Minus Reverse Transcriptase
  • Greater than 96% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities

MSDS & Certificates

pdf   Aminoallyl-dUTP - MSDS

Protocols and Product Manuals

pdf   Aminoallyl-dUTP - Product Information