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Aminoallyl-UTP

Formula: C12H17N3O15P3Na3, Molecular weight: 605.2 (acid form: 539.2)

LO certified

Aminoallyl-UTP is is a modified nucleotide used to enzymatic indirect non-radioactive labeling of RNA during in vitro transcription .
  
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Aminoallyl-UTP chemical structure

Aminoallyl-UTP
Formula: C12H17N3O15P3Na3
Molecular weight: 605.2 (acid form: 539.2)

Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH.

Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.

Applications

  • Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription
  
Storage Condition-20 C
HazardousNo
FormulaC12H17N3O15P3Na3 
General Characteristicslambdamax = 240 nm, extinction coefficient = 11.9 x 103 M-1 x cm-1 (pH 7.0)

lambdamax = 290 nm, extinction coefficient = 7.8 x 103 M-1 x cm-1 (pH 7.0)
Molecular weight605.2 (acid form: 539.2)
Quality Control
  • Functionally tested in in vitro transcription with T7 RNA Polymerase
  • Greater than 96% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities

MSDS & Certificates

pdf   Aminoallyl-UTP - MSDS

Protocols and Product Manuals

pdf   Aminoallyl-UTP - Product Information