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Thermo Scientific Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (see Reference 1) (see Scheme below).
Glucuronidase cleaves X-GlucBCIP-T
Formula: C8H6NO4BrCIP x C7H9NMolecular weight: 433.6
Preparation of a 50 mg/mL stock solution in dimethylformamide is recommended.
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 16.42, 2001).