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BCIP-T

Formula: C8H6NO4BrCIP x C7H9N, Molecular weight: 433.6

Store at -20°C

BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, #R0841) to enhance blue color development.
  
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Thermo Scientific Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (see Reference 1) (see Scheme below).

Applications

  • Detection of alkaline phosphatase activity in:
    • Southern blotting
    • Western blotting
    • Northern blotting
    • Dot/slot blots
    • Plaque and colony screening
BCIP-T

Glucuronidase cleaves X-Gluc
BCIP-T
Formula: C8H6NO4BrCIP x C7H9N
Molecular weight: 433.6

Note

Preparation of a 50 mg/mL stock solution in dimethylformamide is recommended.

Dimerization reaction
  
Storage Condition-20 C
HazardousNo
FormulaC8H6NO4BrCIP x C7H9N
Molecular weight433.6
Quality ControlLess than 3% water content.
Storage NoteStore at -20°C in the dark.

References

  1. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 16.42, 2001).