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Biotin-11-dUTP

Formula: C28H41N6O17P3SNa3, Molecular weight: 927.6 (acid form: 861.6)

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Biotin-11-dUTP is used for enzymatic non-radioactive labelling of DNA.
  
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Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

Applications

  • Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension
Biotin-11-dUTP

Biotin-11-dUTP
Formula: C28H41N6O17P3SNa3
Molecular weight: 927.6 (acid form: 861.6)

  
Storage Condition-20 C
HazardousNo
Extinction Coefficient
  • extinction coefficient = 10.7 x 103 M-1 x cm-1 (pH 7.0)
  • extinction coefficient = 7.1 x 103 M-1 x cm-1 (pH 7.0)
FormulaC28H41N6O17P3SNa3 
lambdamax
  • 240 nm
  • 289 nm
Molecular Weight927.6 (acid form: 861.6)
Quality Control
  • Functionally tested in random primed DNA labeling with Klenow Fragment, exo-
  • Greater than 96% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities

MSDS & Certificates

pdf   Biotin-11-dUTP - MSDS

Protocols and Product Manuals

pdf   Biotin-11-dUTP - Product Information