dNTP/dUTP Mix

PureExtreme dNTPs are manufactured at Thermo Scientific , one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. Thermo Scientific dNTPs are available individually as nucleotide mixes at 2 mM of each dATP, dCTP, dGTP, and 4 mM of dUTP for direct use in PCR with Taq DNA Polymerase, reverse transcription, and other applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA
• Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage:
  • Stable for a minimum 100 freeze-thaw cycles
  • Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
  • Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)
• Application tested in:
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA, and DNA sequencing.

Note

dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.