dNTP Set

Extremly pure dNTPs are manufactured at Thermo Scientific, one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. Thermo Scientific dNTPs are available individually or as a convenient set of 100 mM solutions of each dATP, dTTP, dCTP, and dGTP for direct use in PCR, reverse transcription, labeling, and other applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free ofhuman and E. coli DNA
• Application tested in:
  • Long range PCR (40 kb)
  • cDNA synthesis and RT-PCR
  • Real-time PCR
  • Standard PCR
  • High fidelity PCR
• Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage:
  • Stable after multiple freeze-thaw cycles
  • Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
  • Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)

Applications

• For use in all molecular biology applications, including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling, and DNA sequencing.