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DTT

Formula: C4H10O2S2, Molecular weight: 154.25

Store at 4°C

DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.
  
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DTT

DTT
Formula:
C4H10O2S2
Molecular weight: 154.25

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (see Reference 1).

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

Highlights

  • Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases.

Applications

  • Enzyme stabilization
  • Enzymatic reactions
  • Protein electrophoresis

Note

DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).

  
FormulaC4H10O2S2 
HazardousNo
Molecular weight54.25
Quality Control
  • Absence of endo-, exodeoxyribonucleases, ribonucleases, and proteases confirmed by appropriate quality tests.
  • A ≥ 99.0% reduction of titratable SH groups confirmed by iodometric method.
Storage Condition4 C

References

  1. W. W. Cleland, Dithiothreitol, a new protective reagent for SH groups. Biochemistry. 3, 480-482, 1964.

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