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dUTP

Formula: C9H12N2O14P3Na3, Molecular weight: 534.1 (acid form: 468.1)

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Extremely pure dUTP for molecular biology applications.
  
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Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable and more than 99% pure. It is supplied as 100 mM aqueous solution titrated to pH 7.0 with NaOH. Use dUTP in molecular biology applications.

Highlights

  • Greater than 99% purity confirmed by HPLC
  • Free ofhuman and E. coli DNA
  • Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage
  • Application tested in:
    • Long range PCR (40 kb)
    • cDNA synthesis and RT-PCR
    • Real-time PCR
    • Standard PCR
    • High fidelity PCR

Applications

  • For use in all molecular biology applications, including PCR, real-time PCR and RT-PCR with Taq DNA Polymerase, cDNA synthesis, and primer extension.
dUTP

dUTP
Formula:
C9H12N2O14P3Na3
Molecular weight: 534.1 (acid form: 468.1)

Note

dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.

  
Storage Condition-20 C
HazardousNo
Extinction Coefficient10.0 x 103 M-1 x cm-1 (pH 7.0)
FormulaC9H12N2O14P3Na3 
Lambda<sub>max</sub>262 nm
Molecular weight534.1 (acid form: 468.1)
Quality Control
  • Functionally tested in PCR
  • Greater than 99% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities

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Protocols and Product Manuals