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Exo-Resistant Random Primer

5'-NpNpNpNpNpSNpSN-3'

Store at -20°C

Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
  
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Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases (see Reference 1), such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.

Highlights

  • 3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis

Applications

  • Strand displacement amplification of genomic DNA (see​ Reference 2), plasmids and phage DNA (see Reference 3)
  • DNA labeling by random priming (see​ References 4-6)

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

  
Storage Condition-20 C
HazardousNo
Quality ControlFunctionally tested for the efficient priming of DNA synthesis using phi29 DNA Polymerase.

References

  1. A. Skerra, Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity. Nucleic Acids Res. 20, 3551-3554 (1992).

  2. F. B. Dean et al., Comprehensive human genome amplification using multiple displacement amplification. Proc. Natl. Acad. Sci. 99, 5261-5266 (2002).

  3. F. B. Dean et al., Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res. 11, 1095-1099 (2001).

  4. A. P. Feinberg, B. Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13 (1983).

  5. A. P. Feinberg, B. Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Addendum. Anal. Biochem. 137, 266-267 (1984).

  6. J. Mackey et al., Use of random primer extension for concurrent amplification and nonradioactive labeling of nucleic acids. Anal. Biochem. 212, 428-435 (1993).

MSDS & Certificates

pdf   Exo-Resistant Random Primer - MSDS

Protocols and Product Manuals

pdf   Exo-Resistant Random Primer - Product Information