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Fluorescein-12-dUTP

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Fluorescein-12-dUTP is used for enzymatic non-radioactive labeling of DNA.
  
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Fluorescein-12-UTP chemical structure

Fluorescein-12-UTP
Formula: C39H41N4O21P3
Molecular weight: 994.7

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution. Fluorescein-12-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment and DNA Polymerase I.

Applications

  • Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
  
Storage Condition-20 C
HazardousNo
General CharacteristicsExmax = 495 nm; Emmax = 520 nm (pH 9.0),
lambdamax = 495 nm, extinction coefficient = 70.0 x 103 M-1 x cm-1 (pH 9.0).
Quality Control
  • Functionally tested in cDNA synthesis
  • Greater than 90% purity confirmed by HPLC
  • Free of endo-, exodeoxyribonuclease, ribonuclease, and nicking activities
Storage NoteStore at -20°C in the dark.
Normalized excitation-emission spectra of Fluorescein-12-dUTP

Normalized excitation-emission spectra of Fluorescein-12-dUTP

Normalized excitation-emission spectra of Fluorescein-12-dUTP

Normalized excitation-emission spectra of Fluorescein-12-dUTP.
black – excitation
blue – emission


MSDS & Certificates

pdf   Fluorescein-12-dUTP - MSDS

Protocols and Product Manuals

pdf   Fluorescein-12-dUTP - Product Information