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Thermo Scientific Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed, which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA (see References 1-4).
We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery:
Glycogen, molecular biology grade, recommended for DNA precipitation (#R0561).
Glycogen, RNA grade, can be used for both RNA and DNA precipitation (#R0551).
† At a final concentration up to 8 µg/µL, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling, in vitro transcription, or bacterial transformation. At a final concentration up to 0.4 µg/µL, Glycogen does not affect in vitro transfection of eukaryotic cells with ExGen 500 in vitro Transfection Reagent.
Effect of Glycogen on Precipitation of Nucleic Acids.A 17 nt DNA oligonucleotide was precipitated from 2 pg/µL solution without glycogen and with 1 µg/µL final concentration of Glycogen, molecular biology grade. A 200 bp DNA fragment was precipitated from 50 pg/µL solution without glycogen and with 0.05 µg/µL final concentration of Glycogen, molecular biology grade. 100 nt RNA transcript was precipitated from 50 pg/µL solution, without Glycogen and with 0.05 µg/µL final concentration of Glycogen, RNA grade. All precipitations were performed with 2.5X volumes of ethanol and 0.3 M sodium acetate at room temperature for 5 minutes. The concentration of the nucleic acids was measured spectrophotometrically.