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IPTG

Formula: C9H18O5S, Molecular weight: 238.3

Store at 4°C

IPTG induces the expression of genes under the control of lac operon and is suitable for cloning and protein expression procedures.
  
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Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

Applications

  • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (see Reference 1)
  • Induction of gene expression under control of the lac promoter
IPTG Chemical Structure

IPTG
Formula: C9H18O5S
Molecular weight: 238.3

Note

  • Preparation of a 100 mM (23.83 mg/mL) stock solution in water is recommended
  • For blue/white colony screening use 0.1 mM final IPTG concentration in LB (Luria Broth) media
  
Storage Condition-20 C
HazardousNo
Quality Control
  • Functionally tested in blue/white cloning assay
  • Purity of ≥ 99.0% confirmed by HPLC

References

  1. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.

MSDS & Certificates

pdf   IPTG - MSDS (R0391)
pdf   IPTG Solution - MSDS