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Thermo Scientific IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon (see Reference 1). It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.
Formula: C9H18O5SMolecular weight: 238.3
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.