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NBT

Formula: C40H30N10O6Cl2, Molecular weight: 817.7

Store at 4°C

NBT (nitro blue tetrazolium) is an oxidant used in conjunction with BCIP or BCIP derivatives (BCIP T) to enhance blue color development during detection of alkaline phosphatase activity.
  
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Thermo Scientific NBT is used to enhance blue color development during detection of alkaline phosphatase activity (see Protocols). It is suitable for immunohistochemistry and cytochemistry. NBT is also a substrate for oxidases and dehydrogenases.

Applications

  • Detection of alkaline phosphatase activity in:
    • Southern blotting
    • Western blotting
    • Northern blotting
    • Dot/slot blots
    • Plaque and colony screening
NBT Chemical Structure

NBT
Formula: C40H30N10O6Cl2
Molecular weight: 817.7

Note

Preparation of a 75 g/mL stock solution in 70% dimethylformamide is recommended.

  
Storage Condition4 C
HazardousNo
FormulaC40H30N10O6Cl2
Molecular weight817.7
Quality ControlOne spot by TLC. Less than 8% water content.
StorageStore at 4°C or at -20°C in the dark.

MSDS & Certificates

pdf   NBT - MSDS