Primers for cDNA Synthesis

Available On-Site


Random Hexamer Primer, Oligo(dT)18 Primer, Anchored Oligo dT Primer
  
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Thermo Scientific Random Hexamer Primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready-to-use, 20X concentrated aqueous solution. Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready-to-use, 20X concentrated aqueous solution.

Anchored Oligo-dT primers are specifically designed for synthesising long cDNAs from mRNAs carrying poly-A tails. Since this primer only anneals to the poly-A tail junction of the mRNA template, it eliminates transcription through the poly-A tail, providing more effective cDNA synthesis.

Applications

  • cDNA Synthesis
  • RT-qPCR
  • RT-PCR

Includes

Random Hexamer Primer

  • SO142 contains 24 µg (100 µM)

Oligo(dT)18 Primer

  • SO131 contains 30 µg (100 µM)
  • SO132 contains 60 µg (100 µM)

Anchored Oligo-dT primer

  • AB-1247 contains 500 ng/µL; 100 µL
  
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Storage Condition-20 C
Priming strategies for cDNA Synthesis

Priming strategies for cDNA Synthesis

Priming strategies for cDNA Synthesis

The combination of anchored oligo dT and Random hexamers generates more sensitive and efficient RT-qPCR reactions.


References

Citations