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Formula: C14H15BrClNO6 Molecular weight: 408.6
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).
X-Gal Solution, ready-to-use, is stable, 0.22 µm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, ed. 3, 2001), pp. 1.124-1.125, A1.27.