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X-Gluc

Formula: C14H13BrClNO7 × C6N13N, Molecular Weight: 521.8

Store at -20°C

X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid, cyclohexylammonium salt) is a substrate for β-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
  
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X-Gluc chemical structure

Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Applications

  • Detection of GUS expression in plant cells and tissues (see Reference 1)
  • Detection of infections caused by E. coli
  • Detection of bacterial contamination in food and water samples.
Glucuronidase cleaves X-Gluc

Glucuronidase cleaves X-Gluc

Note

Preparation of a 0.1 M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

  
Storage Condition-20 C
HazardousNo
FormulaC14H13BrClNO7 × C6N13N
Molecular Weight521.8
Quality Control Purity of ≥ 97% confirmed by HPLC
Storage NoteStore at -20°C in the dark.

References

  1. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, ed. 3, 16.42, 2001).

  2. R. Jefferson, Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep. 5, 387-405 (1987).

MSDS & Certificates

pdf   X-Gluc - MSDS