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TranscriptAid T7 High Yield Transcription Kit

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TranscriptAid T7 High Yield Transcription Kit is designed for high yield in vitro transcription from DNA templates containing a T7 RNA Polymerase promoter, including linearized plasmids or PCR products.
  
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The Thermo Scientific TranscriptAid T7 High Yield Transcription Kit  contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours (see Figures 1 and 2). The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA (see Figure 3). All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights

  • Exceptionally high yields – up to 200 µg in 2 hours
  • Versatile – suitable for both short and long RNA transcripts
  • Milligram amounts of RNA in a single, scaled-up reaction
  • Flexible – generates unlabeled, labeled or capped RNA
  • RiboRuler RNA Ladder supplied with kit for sizing and quantification

Applications

  • In vitro transcription
  • In vitro translation
  • Generation of hybridization probes for:
    • microarrays
    • in situ hybridization
    • blotting
  • RNase protection assays
  • RNA binding protein assays
  • Antisense RNA and RNAi
  • RNA amplification
  • Microinjection studies

Includes

  
Storage Condition-20 C
Shelf Life12 Months
HazardousNo
Quality ControlA 20 µL reaction containing 1 µg of the control template DNA generates 140 to 170 µg of intact 2.2 kb RNA transcript after a 2 h incubation.
Time course of RNA synthesis

Time course of RNA synthesis

Time course of RNA synthesis

Figure 1 | Time course of RNA synthesis. 500 b, 1000 b and 6000 b RNA transcripts were generated with TranscriptAid T7 High Yield Transcription Kit. 1 µg of DNA template was used in 20 µL reactions. Yields of RNA were determined at different transcription reaction time points using Agilent 2100 Bioanalyzer®.


Time course of short RNA synthesis

Time course of short RNA synthesis

Time course of short RNA synthesis

Figure 2 | Time course of short RNA synthesis. 50 b and 100 b RNA transcripts were generated with TranscriptAid T7 High Yield Transcription Kit in 0 to 8 hour reaction time. Yields of RNA were determined at different transcription reaction time points using Agilent 2100 Bioanalyzer®.


High yield of full length RNA transcripts

High yield of full length RNA transcripts

High yield of full length RNA transcripts

Figure 3 | High yield of full length RNA transcripts. 500, 1000 and 6000 base RNA transcripts were generated using high yield in vitro transcription reactions containing 1 µg of DNA template. Each 20 µL reaction was incubated for 2 hours at 37°C. The transcription products were diluted 80-fold and 6 µL was run on a 1% agarose/TAE gel supplemented with 0.5 µg/mL ethidium bromide.

M – RiboRuler High Range RNA Ladder, ready-to-use
1, 4, 7 – 500 b RNA transcripts
2, 5, 8 – 1000 b RNA transcripts
3, 6, 9 – 6000 b RNA transcripts