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Precision LentiORFs

Precision LentiORFs are a collection of expression-ready open reading frames (ORFs) cloned into a lentiviral backbone, permitting gene over expression in virtually any cell type.

Enter a common gene identifier and click SEARCH to find products for your gene

Common gene identifiers for your gene can be found at NCBI Gene or miRBase.

Identifier	Description	Examples
Gene ID	The Gene ID is a unique identifying number for each gene in the RefSeq database and tends to be the most stable gene identifier.	672 22421
Accession Number	Accession Numbers are identifiers for a sequence record. The preferred accession numbers for RNAi products are found in the Reference Sequence (RefSeq) database, a non-redundant, curated, and annotated collection of sequence records for major model organisms. RefSeq accession numbers for mRNA begin with the characters NM_ or XM_ followed by six or nine digits. For gene expression products, the GenBank accession number for the clone can also be used for more specific search results.	NM_001798 NM_001170537 BC068303
Gene Symbol	The Gene Symbol can be the official or alternate symbol as displayed on NCBI Gene.	BRCA1 CDK1 CTNNB1
Gene Description	The Gene Description is also known as the Official Full Name on NCBI Gene.	myogenic differentiation 1 thyroid peroxidase breast cancer 1, early onset
miRBase Identifiers	miRBase accession numbers are the most stable identifiers for microRNA. The miRBase ID, or name, may also be used.	miRBase ID: hsa-let-7d, mmu-mir-122 Precurser Accession: MI0000065, MI0000256 Mature Accession: MIMAT0000065, MIMAT0000246

If you require further assistance with your search, please contact Technical Support at ts.molbio@thermofisher.com

Product	Catalog Number
Precision LentiORF Individual cDNA-with native stop codon	OHS5899 2 x 25 µl viral particles - $750.00select
Precision LentiORF Individual cDNA-without native stop codon	OHS5900 2 x 25 µl viral particles - $750.00select
Precision LentiORF Viral Particle Starter Kit	OHS5836 viral particles kit - $1,350.00select
Precision LentiORF RFP Positive Control	OHS5833 viral particles - $327.00select
Precision LentiORF Transduction Starter Kit	OHS5835 glycerol kit - $1,800.00select
Precision LentiORF Transfection Starter Kit	OHS5834 glycerol kit - $1,200.00select
Precision LentiORF Human Library - with and without stop codons (glycerol stock)	OHS6067 1 each - Inquireselect	Inquire
Precision LentiORF Human Library - with native stop codons (glycerol stock)	OHS6069 1 each - Inquireselect	Inquire
Precision LentiORF Human Library - without native stop codons (glycerol stock)	OHS6068 1 each - Inquireselect	Inquire

Description
Specifications
Supporting Data
References
Resources

Thermo Scientific Precision LentiORFs are fully-sequenced human open reading frames (ORFs) derived from cDNA coding sequences. These ORFs have been cloned into a lentiviral backbone, enabling gene and protein expression experiments in virtually any mammalian cell type.

Features of the Precision LentiORF backbone include a CMV promoter for robust protein expression, a GFP reporter to visually track protein expression, and blasticidin resistance for selection of stable cell lines. Precision LentiORF clones are available with or without native stop codons, and secondary tags can be added to the MultiTag cloning site located downstream of the coding sequence.

Highlights

Lentiviral ORF constructs require no subcloning or Gateway recombination
Fully sequenced coding sequences
GFP reporter is transported to the nuclease for easy visualization of expression
Available as ready-to-transduce viral particles, glycerol stocks, or LentiORF Starter Kits

Applications

Potential applications of Precision LentiORFs include gene modulation experiments, RNAi rescue (see the RNAi Rescue using Precision LentiORF technical note), and expression of human protein in virtually any mammalian cell-type. Transduce primary and non-dividing cells with ready-to-use lentiviral particles, or transfect Precision LentiORF constructs directly into your cell line. For generating your own viral particles, Trans-Lentiviral ORF packaging kits are also available.

Formats

Individual Clones – Glycerol Stock
Viral Particles – provided as 2 x 25 µL aliquots with a minimum titer of 10^8 TU/mL
LentiORF Starter Kits – Choose from transfection kits, transduction kits, and viral particle kits

Precision LentiORF Viral Particle starter kits include:

Two Precision LentiORF constructs provided as 2 x 25µL aliquots of high-titer viral particles (1 x 10^8 TU/mL)
Precision LentiORF RFP control provided as 25 µL of high-titer virus

Precision LentiORF Transduction starter kits include:

Two Precision LentiORF constructs
Trans-Lentiviral packaging mix sufficient for 10 packaging events
Calcium Phosphate transfection reagent
Precision LentiORF RFP control

Precision LentiORF Transfection starter kits include:

Two Precision LentiORF constructs
TurboFect transfection reagent
Precision LentiORF RFP control

Note

The Precision LentiORF expression vectors are not compatible with third generation packaging systems. We recommend the Trans-Lentiviral Packaging System for use with these vectors.


Shipping Condition	Wet Ice,Dry Ice,Ambient
Storage Condition	-80 C
Shelf Life	12 Months,13 Months
Hazardous	No

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.

Clone Collections

Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.