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Lambda DNA

Store at -20°C

Lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. The DNA is linear and double-stranded.
  
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Thermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.

Features

Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is often used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. The map shows enzymes that cut lambda DNA once. Enzymes produced by Thermo Scientific are shown in orange (see figure bellow).

Applications

  • Activity and specificity assays of restriction enzymes
  • Preparation of DNA molecular weight standards
  • Cloning
Lambda DNA map

Phage Lambda DNA map. Download Lambda sequence in FASTA, GenBank, or EMBL format.

GenBank/EMBL Accession Numbers

J02459, M17233, M24325, V00636, X00906.

  
CategoryName
Concentration0.3 µg/µL
HazardousNo
Hazardous:No
Quality Control
  • DNA concentration is confirmed spectrophotometrically.
  • Identity and homogenity of DNA is confirmed by digestion by CpoI and HindIII, and analysis of DNA fragmentation patterns.
REviewerUse for DNA sequence analysis and map creation.
Shelf Life:
Shipping Condition:
Shipping Information
SpecificationName
SpecificationValue
Storage Buffer10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
Storage Condition-20 C
Storage Condition:

References

  1. D. L. Daniels et al., Appendix II: Complete annotated lambda sequenceR. W. Hendrix, J. W. Roberts, F. W.Stahl, and R. A. Weisberg, Eds. (Cold Spring Harbor Laboratory, Cold Spring Harbor, 1983), pp. 519-674. [LAMBDA II]

  2. D. L. Daniels et al.Appendix I: A molecular map of coliphage lambda, R. W. Hendrix, J. W. Roberts, F. W.Stahl, and R. A. Weisberg, Eds. (Cold Spring Harbor Laboratory, Cold Spring Harbor, 1983), pp. 469-517. [LAMBDA II]

  3. Sanger, F., et al., Nucleotide sequence of bacteriophage lambda DNA, J. Mol. Biol. ,162, 729-773, 1982.

Citations

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