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M13/pUC reverse sequencing primer (-26), 17-mer

5'-d(CAGGAAACAGCTATGAC)-3'

Store at -20°C

M13 pUC sequencing primers are single-stranded oligonucleotides used for sequencing of DNA fragments inserted into the MCS of various pUC19-based cloning vectors.
  
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The Thermo Scientific M13/pUC sequencing primers anneal to the region in the 5'-terminus of the lacZ gene. All primers are supplied as 10 µM aqueous solutions.

Applications

  • Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19, pTZ19R, pTZ57R, M13mp18, and pBluescript II
  • Colony screening by PCR
  
Storage Condition-20 C
HazardousNo
Quality Control

Functionally tested by dideoxy sequencing of pUC19 DNA.