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Thermo Scientific pBR322 is one of the most commonly used E.coli cloning vectors.
pBR322 DNA map. Download pBR322 sequence in FASTA, GenBank, or EMBL format.
pBR322 is 4361 bp in length and contains:
The circular sequence is numbered such that 1 is the first T of the unique EcoRI site GAATTC and numbering increases through the tet gene and the pMB1 material and finally through the Tn3 region.
The map shows enzymes that cut pBR322 DNA once. Enzymes produced by Thermo Scientific are shown in orange. The coordinates refer to the position of the first nucleotide in each recognition sequence.
The exact positions of the genetic elements are shown on the map (termination codons included). The bla gene nucleotides 4153-4085 (complementary strand) code for a signal peptide. The indicated rep region is sufficient to promote replication. DNA replication initiates at position 2533 (± 1) and proceeds in the direction indicated. Plasmids carrying the pMB1 and ColE1 replicons are incompatible, but they are fully compatible with those carrying the p15A replicon (pACYC177, pACYC184). pMB1-derived plasmids can be amplified using chloramphenicol.
J01749, K00005, L08654, M10282, M10283, M10286, M10356, M10784, M10785, M10786, M33694, V01119
F. Bolivar et al., Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 2, 95-113 (1977).
L. Covarrubias et al., Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene. 13, 25-35 (1981).
K. W. Peden, Revised sequence of the tetracycline-resistance gene of pBR322. Gene. 22, 277-280 (1983).
J. G. Sutcliffe, Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc. Natl. Acad. Sci. USA. 75, 3737-3741 (1978).
J. G. Sutcliffe, Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb. Symp. Quant. Biol. 43(Pt 1), 77-90 (1979).
N. Watson, A new revision of the sequence of plasmid pBR322. Gene. 70, 399-403 (1988).