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pBR322 DNA

Store at -20°C

pBR322 DNA is a 4361 bp double-stranded closed circular medium copy plasmid DNA commonly used for cloning.
  
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Thermo Scientific pBR322 is one of the most commonly used E.coli cloning vectors.

Highlights

  • Isolated from E. coli (dam+, dcm+)
  • More than 90% in the supercoiled form
  • Purified by chromatography using proprietary patented technology
  • For DNA sequence, sequence analysis, and map creation, see the REviewer online free tool.

Applications

  • Cloning
  • Preparation of DNA molecular weight standards
pBR322 Plasmid Map

pBR322 DNA map. Download pBR322 sequence in FASTA, GenBank, or EMBL format.

Click here for pBR322 contents and usage notes

  
CategoryName
HazardousNo
Hazardous:No
Quality Control
  • DNA concentration is confirmed spectrophotometrically.
  • Identity and homogenity of DNA is confirmed by digestion by AluI, BsuRI, and HindIII, and analysis of DNA fragmentation patterns.
REviewer Use for DNA sequence analysis and map creation.
Shelf Life:
Shipping Condition:
Shipping Information
SpecificationName
SpecificationValue
Storage Buffer10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
Storage Condition-20 C
Storage Condition:
pBR322 DNA map

pBR322 DNA map

pBR322 DNA map


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