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Thermo Scientific pUC18 and pUC19 vectors are small, high copy number, E. coli plasmids, 2686 bp in length. They are identical except that they contain multiple cloning sites (MCS) arranged in opposite orientations.
pUC18/pUC19 Plasmid map.
Download pUC18 sequence in FASTA, GenBank, or EMBL format.
Download pUC19 sequence in FASTA, GenBank, or EMBL format.
The map shows enzymes that cut pUC18/19 DNA once. Enzymes produced by Thermo Scientific are shown in orange. The coordinates refer to the position of the first nucleotide in each recognition sequence.
The exact positions of the genetic elements are shown on the map (termination codons included). The bla gene nucleotides 2486-2418 (complementary strand) code for a signal peptide. The LacZ polypeptide corresponding to wt beta-galactosidase and essential for blue/white screening ends at nt position 236 (compl. strand). Another 30 codons in the same reading frame are derived from pBR322. The indicated rep region is sufficient to promote replication. DNA replication initiates at position 866 (± 1) and proceeds in the direction indicated. Plasmids carrying the pMB1 and ColE1 replicons are incompatible, but they are fully compatible with those carrying the p15A replicon (pACYC177, pACYC184). pMB1-derived plasmids can be amplified using chloramphenicol.
C. Yanisch-Perron, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33, 103-119 (1985).