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pUC57 DNA

Store at -20°C

pUC57 DNA is a double-stranded closed circular high copy plasmid DNA used in cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII.
  
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Thermo Scientific Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3’-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (±1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.

Highlights

  • More than 90% in the supercoiled form
  • Isolated from E. coli (dam+, dcm+)
  • Purified by chromatography using proprietary patented technology

Applications

  • Cloning
  • Sequencing of insert DNA
  • Generation of nested deletions with ExoIII
pUC57 plasmid map

pUC57 Plasmid map. Download pUC18 sequence in FASTA, GenBank, or EMBL format.

For DNA sequence, sequence analysis and map creation, see the free online REviewer tool.

  
GenBank/EMBL Accession NumberY14837
HazardousNo
Plasmid loci
  • CAP protein binding site – 615-578 (compl. strand)
  • mRNA (LacZ) starts at nt position 531 (compl. strand)
  • lac repressor binding site – 531-511 (compl. strand)
Quality Control
  • DNA concentration is confirmed spectrophotometrically.
  • Identity and homogenity of DNA is confirmed by digestion by NdeI+HindIII and HindIII, and analysis of DNA fragmentation patterns.
Storage Buffer10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
Storage Condition-20 C

References

  1. C. Yanisch-Perron et al., Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33, 103-119 (1985).

Citations