Yeast TAP Tagged ORFs

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Yeast-TAP Tagged ORF library allows the purification and the selection of the yeast proteome and associated components using two affinity selection steps in tandem.
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Common gene identifiers for your gene can be found at NCBI Gene or miRBase.

IdentifierDescriptionExamples
Gene IDThe Gene ID is a unique identifying number for each gene in the RefSeq database and tends to be the most stable gene identifier.
  • 672
  • 22421
Accession NumberAccession Numbers are identifiers for a sequence record. The preferred accession numbers for RNAi products are found in the Reference Sequence (RefSeq) database, a non-redundant, curated, and annotated collection of sequence records for major model organisms. RefSeq accession numbers for mRNA begin with the characters NM_ or XM_ followed by six or nine digits. For gene expression products, the GenBank accession number for the clone can also be used for more specific search results.
  • NM_001798
  • NM_001170537
  • BC068303
Gene SymbolThe Gene Symbol can be the official or alternate symbol as displayed on NCBI Gene.
  • BRCA1
  • CDK1
  • CTNNB1
Gene DescriptionThe Gene Description is also known as the Official Full Name on NCBI Gene.
  • myogenic differentiation 1
  • thyroid peroxidase
  • breast cancer 1, early onset
miRBase IdentifiersmiRBase accession numbers are the most stable identifiers for microRNA. The miRBase ID, or name, may also be used.
  • miRBase ID: hsa-let-7d, mmu-mir-122
  • Precurser Accession: MI0000065, MI0000256
  • Mature Accession: MIMAT0000065, MIMAT0000246

If you require further assistance with your search, please contact Technical Support at ts.molbio@thermofisher.com

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ProductCatalog Number 
TAP Tagged strain (endogenous promoter)
YSC1178 glycerol stock - $75.00select
Yeast TAP-Fusion Membrane Protein Subset
YSC1247 glycerol stock - $838.00select
TAP Tagged ORF Collection
YSC1177 glycerol stock - $3,978.00select

The Yeast-TAP Tagged ORF library allows the purification and the selection of the yeast proteome and associated components using two affinity selection steps in tandem, enabling the development of a range of high-throughput functional assays. By tagging each ORF with a high-affinity epitope and expressing the protein from its natural chromosomal location, a census of proteins expressed during log-phase growth can be quantified through immunodetection of the common tag.

The C-terminal TAP insertion cassette contains the coding region for a modified version of the TAP (Tandem Affinity Purification) tag, which consists of a calmodulin binding peptide, a TEV cleavage site, and two IgG binding domains of Staphylococcus aureus protein A as well as a selectable marker.

Highlights

  • Allows for the purification and the selection of the yeast proteome and associated components using two simple affinity selection steps in tandem
  • A membrane-bound protein subset has been arrayed in separate plates for your convenience
  • Background strain = MatA (BY4741)
Vector Schematic of the Yeast TAP-Tagge Collection.

The C-terminal TAP insertion cassette contains the coding region for a modified version of the TAP (Tandem Affinity Purification) tag, which consists of a calmodulin binding peptide, a TEV cleavage site, two IgG binding domains of Staphylococcus aureus protein A, and a selectable marker.

Note

We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.

Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

  
HazardousNo
Shelf Life12 Months
Shipping ConditionDry Ice,Wet Ice
Storage Condition-80 C

Individual strains are provided in tubes containing glycerol stock cultures in YPD plus 15% glycerol. While the scTAP strains are supplied in YPD media, SD complete or SD -HIS media can also be used. Individual strains from this library can be queried and purchased through our clone query.

The TAP Library is provided in 96-well microtiter plates containing frozen glycerol stock cultures in YPD plus 15% glycerol. While the scTAP strains are supplied in YPD media, SD complete or SD-HIS media can also be used. Strains are arranged in plates by protein expression size from largest to smallest, with plate designation GS1 representing high expression and GS5 representing low expression. In addition, the membrane-protein subset has been arrayed in separate plates for your convenience. Refer to CD shipped with the collection for expression category designation.

The use of the TAP-tag for the purification of biomolecule/protein complexes is covered by granted patents in Europe and Australia (EP1105508B1, AU07629621), and patent applications in Canada, Japan, and US (see International Patent Publication No. WO-0009716).

For licensing information for use in purification of biomolecule/protein complexes, please contact Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany, at info@cellzome.com.

Yeast Tap Fusion - 1

Yeast Tap Fusion - 1

Yeast Tap Fusion - 1

The abundance of proteins ranged from fewer than 50 to more than 106 molecules/cell with many, including essential proteins and most transcription factors, having levels not readily detectable by other proteomic techniques, nor predictable by mRNA levels or codon bias measurements.


Yeast Tap Fusion - 2

Yeast Tap Fusion - 2

Yeast Tap Fusion - 2

The abundance of proteins ranged from fewer than 50 to more than 106 molecules/cell with many, including essential proteins and most transcription factors, having levels not readily detectable by other proteomic techniques, nor predictable by mRNA levels or codon bias measurements.


References

  1. S. Ghaemmaghami et al., Global Analysis of Protein Expression in Yeast. Nature. 425(6959), 737-741 (16 October 2003). [Letters to Nature]

Citations