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2X RNA Loading Dye

Store at 4°C

2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels.
  
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Electrophoresis of tracking dyes in 2X RNA Loading Dye

Thermo Scientific 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide. In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.

2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilizes RNA.

Highlights

  • Two-color tracking of DNA, RNA migration during electrophoresis
  • No DNA, RNA masking during gel exposure to UV light
  • Denaturation of DNA, RNA due to the presence of formamide

Applications

  • Preparation of DNA ladders, markers, and samples for loading on denaturing gels
  • Preparation of RNA ladders, markers, and samples for loading on agarose or polyacrylamide gels
  
Storage Condition-20 C
HazardousNo
Composition
  • 95% formamide
  • 0.025% SDS
  • 0.025% bromophenol blue
  • 0.025% xylene cyanol FF
  • 0.025% ethidium bromide
  • 0.5 mM EDTA
Quality Control
  • Tested in RNA sample preparation prior to agarose gel electrophoresis.
  • The absence of ribonucleases is confirmed by appropriate tests.
StorageStore at 4°C up to 12 months. For longer periods, store at -20°C.