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50X TAE Buffer (Tris-acetate-EDTA)

Store at room temperature

50X TAE Buffer (Tris-acetate-EDTA) is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
  
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Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Applications

  • Electrophoresis of nucleic acids in agarose and polyacrylamide gels
  • Used both as a running buffer and as a gel preparation buffer
  • Filtered through a 0.22 µm membrane
  • Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA

Note

TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.

  
Storage ConditionAmbient
HazardousNo
1X Composition40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4
Quality ControlThe absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.
StorageThere are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.
Usage Recommendations
  • Use fresh 1X TAE both for the gel and for the electrophoresis run.
  • To prepare 1X TAE buffer add 20 mL of 50X TAE buffer to 980 mL of deionized water and mix well.