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6X DNA Loading Dye & SDS Solution

Store at -20°C

DNA loading dye containing SDS is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins.
  
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Electrophoresis of tracking dyes in 6X DNA Loading Dye & SDS Solution 

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

  • 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
  • 100 mM EDTA inhibits metal-dependent nucleases

Applications

  • Analysis of DNA samples with high amounts of DNA binding proteins
  • Kinetic experiments
  • DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation reactions
  
Storage Condition-20 C
HazardousNo
6X Solution Composition
  • 0.03% bromophenol blue
  • 0.03% xylene cyanol FF
  • 60% glycerol
  • 1% SDS
  • 100 mM EDTA (pH 7.6, adjusted with Tris)
Quality Control
  • Tested for DNA sample preparation prior to agarose gel electrophoresis.
  • The absence of deoxyribonucleases confirmed by appropriate tests.
StorageStore at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.
Tracking dye migration in agarose gels

Tracking dye migration in agarose gels

Tracking dye migration in agarose gels

Tracking dye migration in agarose gels.