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6X DNA Loading Dye

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Store at -20°C

6X DNA Loading Dye
6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.
  
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Electrophoresis of tracking dyes in 6X DNA Loading Dye

Thermo Scientific 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases.

6X DNA Loading Dye is used for conventional DNA electrophoresis (see Recommendations for use).

Highlights

  • Two-color tracking of DNA migration during DNA electrophoresis
  • No DNA masking during gel exposure to UV light
  • EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

  • Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels
  
Composition
  • 10 mM Tris-HCl (pH 7.6)
  • 0.03% bromophenol blue
  • 0.03% xylene cyanol FF
  • 60% glycerol
  • 60 mM EDTA
HazardousNo
Quality Control
  • Tested for DNA sample preparation prior to agarose gel electrophoresis.
  • The absence of deoxyribonucleases confirmed by appropriate tests.
StorageStore at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.
Storage Condition-20 C
Tracking dye migration in agarose gels

Tracking dye migration in agarose gels

Tracking dye migration in agarose gels

Tracking dye migration in agarose gels.


References

Citations