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6X Orange DNA Loading Dye

Store at -20°C

6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.
  
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Electrophoresis of tracking dyes in 6× Orange DNA Loading Dye 

Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X Orange DNA Loading Dye is used for conventional DNA electrophoresis (see Recommendations for use).

Highlights

  • Two-color tracking of DNA migration during DNA electrophoresis 
  • No DNA masking during gel exposure to UV light
  • EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

  • Analysis of small DNA molecules
  • Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels
  
Storage Condition-20 C
HazardousNo
Composition
  • 10 mM Tris-HCl (pH 7.6)
  • 0.15% orange G
  • 0.03% xylene cyanol FF
  • 60% glycerol
  • 60 mM EDTA
Quality Control
  • Tested for DNA sample preparation prior to agarose gel electrophoresis.
  • The absence of deoxyribonucleases confirmed by appropriate tests.
StorageStore at room temperature or at 4°C up to 12 months. For longer periods, store at -20°C.