Home | Molecular Biology | Molecular Biology Applications | Molecular Cloning | DNA Ladders

FastRuler Ultra Low Range DNA Ladder, Ready-to-Use, 10-200 bp

rt.gif

FastRuler DNA Ladders are specifically designed for fast sizing and approximate quantification of dsDNA in high throughput gels and conventional agarose gels.
  
Loading...
FastRuler Ultra Low Range DNA Ladder, ready-to-use. 4% TopVision LE GQ Agarose (#R0491). 20 µL/lane, 1X TBE, 7 V/cm, 14 min. 

FastRuler Ultra Low Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

The ladder is especially useful for electrophoresis of PCR products.

FastRuler Ultra Low Range DNA Ladder, ready-to-use contains dephosphorylated DNA fragments and is ideal for forward 5-end labeling with T4 Polynucleotide Kinase (see the Certificate of Analysis for the detailed labeling protocol).

Highlights

  • Fast separation (8 to 14 min)
  • Short separation distance (10 to 20 mm)
  • Sharp bands, easy-to-remember fragment sizes and quantities
  • Ready-to-use – premixed with loading dye
  • Supplied with loading dye solution for sample DNA 
  
Storage Condition-20 C
HazardousNo
6× Orange DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Bands
  • 200 bp
  • 100 bp
  • 50 bp
  • 20 bp
  • 10 bp
Quality Control
  • Well-defined bands are formed during 8 to 14 min agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.
FastRuler Ultra Low Range DNA Ladder, ready-to-use

FastRuler Ultra Low Range DNA Ladder, ready-to-use

FastRuler Ultra Low Range DNA Ladder, ready-to-use

FastRuler Ultra Low Range DNA Ladder, ready-to-use.
a. Time course of band separation. Electrophoresis conditions: 20 µL/lane, 4% TopVision LE GQ Agarose, 1X TBE, 7 V/cm.
b. Performance in 96-well format gel electrophoresis. Electrophoresis conditions: 10 µL/lane, 4% TopVision LE GQ Agarose, 1X TBE, 7 V/cm, 14 min.
1 – 200 bp
2 – 150 bp
3 – 100 bp
4 – 75 bp
5 – 50 bp
6 – 35 bp
7 – 25 bp
M – FastRuler Ultra Low Range DNA Ladder, ready-to-use