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FastRuler High Range DNA Ladder, Ready-to-Use, 500-10,000 bp

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FastRuler DNA Ladders are specifically designed for fast sizing and approximate quantification of dsDNA in high throughput gels and conventional agarose gels.
  
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FastRuler High Range DNA Ladder, ready-to-use. 1% TopVision LE GQ Agarose (#R0491). 20 µL/lane, 1X TAE, 7 V/cm, 14 min. 

The FastRuler High Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

The ladder is especially useful for electrophoresis of PCR products.

Highlights

  • Fast separation (8 to 14 min)
  • Short separation distance (10 to 20 mm)
  • Sharp bands, easy-to-remember fragment sizes and quantities
  • Ready-to-use – premixed with loading dye
  • Supplied with loading dye solution for sample DNA 
  
Storage Condition-20 C
HazardousNo
6× MassRuler DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol, and 60 mM EDTA.
Bands
  • 10,000 bp
  • 4,000 bp
  • 2,000 bp
  • 1,000 bp
  • 500 bp
Quality Control
  • Well-defined bands are formed during 8 to 14 min agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Storage and Loading Buffer10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
Time course of FastRule High Range DNA Ladder band separation

Time course of FastRule High Range DNA Ladder band separation

Time course of FastRule High Range DNA Ladder band separation

FastRuler High Range DNA Ladder, ready-to-use.
a. Time course of band separation. Electrophoresis conditions: 20 µL/lane, 1% TopVision LE GQ Agarose, 1X TAE, 7 V/cm.
b. Performance in 96-well format gel electrophoresis. Electrophoresis conditions: 10 µL/lane, 1% TopVision LE GQ Agarose, 1X TAE, 7 V/cm, 14 min.
1 – 10,000 bp
2 – 7,000 bp
3 – 5,000 bp
4 – 4,000 bp
5 – 3,000 bp
6 – 2,000 bp
7 – 1,000 bp
M – FastRuler High Range DNA Ladder, ready-to-use