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FastRuler Middle Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.
The ladder is especially useful for electrophoresis of PCR products.
FastRuler Middle Range DNA Ladder, ready-to-use.a. Time course of band separation. Electrophoresis conditions: 20 µL/lane, 1% TopVision LE GQ Agarose, 1X TAE, 7 V/cm.b. Performance in 96-well format gel electrophoresis. Electrophoresis conditions: 10 µL/lane, 1% TopVision LE GQ Agarose, 1X TAE, 7 V/cm, 14 min.1 – 5,000 bp2 – 3,000 bp3 – 2,000 bp4 – 1,500 bp5 – 1,000 bp6 – 700 bp7 – 500 bpM – FastRuler Middle Range DNA Ladder, ready-to-use