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GeneRuler 1 kb DNA Ladder 250 to 10,000 bp


GeneRuler 1kb DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
  
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GeneRuler 1K DNA DNA Ladder, ready-to-use. 4%TopVision LE GQ Agarose (#R0491). 0.5µg/lane, 8cm length gel, 1×TAE, 7V/cm, 45min. 

Thermo Scientific GeneRuler 1kb DNA Ladder, range 250 to 10,000bpcan be labeled radioactively with T4 Polynucleotide Kinase (seeProtocol).

The ladder is supplied with 6X DNA Loading Dye, and it can be used in DNA sizing and approximate quanitification applications.

Highlights

  • Ideal for both DNA sizing and approximate quantification
  • Sharp bands.
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA
  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol and 60mM EDTA.
Bands10,000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1000, 750, 500, and 250bp
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
StorageStore at -20°C.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA

Protocols and Product Manuals

pdf   GeneRuler 1 kb DNA Ladder 250 to 10,000 bp - Product Information (SM0312)
pdf   General Recommendations for DNA Electrophoresis Protocol
pdf   Recommendations for Accurate Gel Quantification Protocol
pdf   Alkaline Agarose Gel Electrophoresis - Protocol
pdf   Non-denaturing Agarose Gel Electrophoresis Protocol
pdf   Preparation of DNA Ladders/Markers for Electrophoresis Protocol
pdf   GeneRuler 1 kb DNA Ladder 250 to 10,000 bp - Product Information (SM0311)
pdf   Preparation of DNA Samples for Conventional DNA Electrophoresis
pdf   Preparation of DNA Samples from Enzymatic Reaction Mixtures or with Samples Containing High Amounts of DNA Binding Proteins - Protocol
pdf   DNA/RNA 5'-end Labeling by T4 Polynucleotide Kinase in the Forward Reaction Protocol
pdf   Phosphorylation of DNA with T4 Polynucleotide Kinase
pdf   SM0311 GeneRuler 1 kb DNA Ladder - Product Information
pdf   SM0312 GeneRuler 1 kb DNA Ladder - Product Information