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GeneRuler 1 kb Plus DNA Ladder 75 to 20,000 bp


GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels.
  
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GeneRuler 1 kb Plus DNA Ladder - Bands visible at 20,000, 10,000, 7000, 5000, 4000, 3000, 2000, 1500, 1000, 700, 500, 400, 300, 200 and 75 bp. 

Thermo Scientific GeneRuler 1 kb Plus DNA Ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler 1 kb Plus DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (see Protocol).

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
Bands20,000, 10,000, 7000, 5000, 4000, 3000, 2000, 1500, 1000, 700, 500, 400, 300, 200, and 75 bp
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.