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GeneRuler 50 bp DNA Ladder, 50-1000 bp


DNA ladder for sizing and for approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
  
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GeneRuler 50bp DNA Ladder

Thermo ScientificGeneRuler 50bp DNA Ladder is recommended for sizing and for approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler 50bp DNA Ladder can be labeled radioactively with T4Polynucleotide Kinase (see Resources for protocol).

The ladder is supplied with 6X DNA Loading Dye.

Highlights

  • Ideal for both DNA sizing and approximate quantification
  • Sharp bands
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA
  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA.

Protocols and Product Manuals

pdf   GeneRuler 50 bp DNA Ladder - Product Information
pdf   Alkaline Agarose Gel Electrophoresis - Protocol
pdf   General Recommendations for DNA Electrophoresis Protocol
pdf   Non-denaturing Agarose Gel Electrophoresis Protocol
pdf   Preparation of DNA Ladders/Markers for Electrophoresis Protocol
pdf   Recommendations for Accurate Gel Quantification Protocol
pdf   Preparation of DNA Samples for Conventional DNA Electrophoresis
pdf   Preparation of DNA Samples from Enzymatic Reaction Mixtures or with Samples Containing High Amounts of DNA Binding Proteins - Protocol
pdf   DNA/RNA 5'-end Labeling by T4 Polynucleotide Kinase in the Forward Reaction Protocol
pdf   Phosphorylation of DNA with T4 Polynucleotide Kinase
pdf   SM0371 GeneRuler 50 bp DNA Ladder - Product Information
pdf   SM0372 GeneRuler 50 bp DNA Ladder - Product Information