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GeneRuler Express DNA Ladder 100-5000 bp


GeneRuler Express DNA Ladder is designed for fast separation (in 5 to 15minutes at 23V/cm) of double-stranded DNA fragments in agarose gels.
  
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GeneRuler Express DNA Ladder 

Thermo Scientific GeneRuler Express DNA Ladder is designed for fast separation (in 5 to 15minutes at 23V/cm) of double-stranded DNA fragments in agarose gels (see Figure 1 and Table 1 in Supporting Data). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Express DNA Ladder can be labeled radioactively with T4Polynucleotide Kinase (see protocol in Resources).

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Ideal for fast separation under a wide range of electrophoresis conditions 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA.
Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage (23 V/cm).

Electrophoresis conditions: 0.5 µg/lane, 1% TopVision LE GQ Agarose, 1X TAE, 23 V/cm, 5 to 15 minutes.


GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder Separation Guide for Various Electrophoresis Conditions.