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Thermo Scientific GeneRuler Express DNA Ladder is designed for fast separation (in 5 to 15minutes at 23V/cm) of double-stranded DNA fragments in agarose gels (see Figure 1 and Table 1 in Supporting Data). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler Express DNA Ladder can be labeled radioactively with T4Polynucleotide Kinase (see protocol in Resources).
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Fast separation of GeneRuler Express DNA Ladder at high voltage (23 V/cm).
Electrophoresis conditions: 0.5 µg/lane, 1% TopVision LE GQ Agarose, 1X TAE, 23 V/cm, 5 to 15 minutes.
GeneRuler Express DNA Ladder Separation Guide for Various Electrophoresis Conditions.