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GeneRuler Express DNA Ladder, Ready-to-Use

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DNA Ladder designed for fast separation (in 5 to 15 minutes at 23  V/cm) of double-stranded DNA fragments in agarose gels. Premixed with Loading Dye for direct loading onto gel.
  
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GeneRuler Express DNA Ladder<br/>O'GeneRuler Express DNA Ladder, ready-to-use 

Thermo Scientific GeneRuler Express DNA Ladder, ready-to-use, is designed for fast separation (in 5 to 15 minutes at 23 V/cm) of double-stranded DNA fragments in agarose gels (see Figure 1 and Table 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage, or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Express DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose gels.

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Ideal for fast separation under a wide range of electrophoresis conditions 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Can be directly loaded and are stable at room temperature for 6 months 
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6X DNA Loading Dye10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.
GeneRuler Storage and Loading Buffer (for ready-to-use ladders)10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF, and 10% glycerol.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage NotesStore at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage

Fast separation of GeneRuler Express DNA Ladder at high voltage (23 V/cm).

Electrophoresis conditions: 0.5 µg/lane, 1% TopVision LE GQ Agarose, 1X TAE, 23 V/cm, 5 to 15 minutes.


GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder separation guide for various electrophoresis

GeneRuler Express DNA Ladder Separation Guide for Various Electrophoresis Conditions.