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GeneRuler High Range DNA Ladder 10,171 to 48,502 bp


The ladder is recommended for fast sizing and approximate quantification of high molecular weight double-stranded DNA on agarose gels.
  
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GeneRuler High Range DNA Ladder - 48,502, 24,508, 20,555, 17,000, 15,258, 13,825, 12,119 and 10,171 bands. 

Thermo Scientific GeneRuler High Range DNA Ladder is recommended for fast sizing and approximate quantification of high molecular weight double-stranded DNA on agarose gels (in 1.5hour, 0.4%agarose gel). The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler High Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (seeProtocol in the Resources.)

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Ideal for fast sizing of high molecular weight DNA fragments 
  • Sharp bands 
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6× DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60 mM EDTA.
Bands48,502, 24,508, 20,555, 17,000, 15,258, 13,825, 12,119, and 10,171 bands.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA.