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GeneRuler Low Range DNA Ladder 25 to 700 bp


The ladder is recommended for fast sizing and approximate quantification of low molecular weight double-stranded DNA on agarose gels.
  
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GeneRuler Low Range DNA Ladder - 700, 500, 400, 300, 200, 150, 100, 75, 50 and 25bp bands. 

Thermo Scientific GeneRuler Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA fragments on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (seeProtocol).

The ladder is supplied with 6X DNA Loading Dye.

Highlights

  • Ideal for both DNA sizing and approximate quantification 
  • Ideal for non-denaturing polyacrylamide gel electrophoresis 
  • Sharp bands 
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA 
  
Storage Condition-20 C
HazardousNo
6× DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Bands700, 500, 400, 300, 200, 150, 100, 75, 50, and 25bp bands.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA.