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GeneRuler Ultra Low Range DNA Ladder 10 to 300 bp


The ladder is recommended for sizing and approximate quantification of small dsDNA fragments on agarose or polyacrylamide gels. Ideal for siRNA analysis.
  
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GeneRuler Ultra Low Range DNA Ladder - 300, 200, 150, 100, 75, 50, 35, 25, 20, 15 and 10bp bands.

Thermo Scientific GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler Ultra Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (seeProtocol).

The ladder is recommended for analysis of siRNA (see Figure 1)

The ladder is supplied with 6X DNA Loading Dye for sample DNA.

Highlights

  • Ideal for both DNA sizing and approximate quantification
  • Ideal for non-denaturing polyacrylamide gel electrophoresis
  • Ideal for analysis of siRNA
  • Sharp bands
  • Bright reference bands (given in red)
  • Supplied with loading dye for sample DNA
  
Storage Condition-20 C
HazardousNo
6× DNA Loading Dye10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA.
Bands300, 200, 150, 100, 75, 50, 35, 25, 20, 15, and 10bp bands.
Quality Control
  • Well-defined bands are formed during agarose gel electrophoresis.
  • The DNA concentration is determined spectrophotometrically.
  • The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)10mM Tris-HCl (pH7.6) and 1mM EDTA.
siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

siRNA analysis by GeneRuler/O'GeneRuler Ultra Low Range DNA ladders

Figure 1 | siRNA gel analysis by GeneRuler and O'GeneRuler Ultra Low Range DNA ladders
Electrophoresis conditions: 5% agarose gel, 1X TBE buffer, 7 V/cm, 45 min.
1 – GeneRuler Ultra Low Range DNA Ladder
2 – 21 bp siRNA (0.5 µg), premixed with 6X DNA Loading Dye