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Thermo Scientific GeneRuler Ultra Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler Ultra Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (seeProtocol).
The ladder is recommended for analysis of siRNA (see Figure 1)
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Figure 1 | siRNA gel analysis by GeneRuler and O'GeneRuler Ultra Low Range DNA laddersElectrophoresis conditions: 5% agarose gel, 1X TBE buffer, 7 V/cm, 45 min.1 – GeneRuler Ultra Low Range DNA Ladder2 – 21 bp siRNA (0.5 µg), premixed with 6X DNA Loading Dye